Regulation of Listeria monocytogenes internalin-like genes by Ï?B and PrfA as revealed by subgenomic microarray analyses

ABSTRACT: The Listeria monocytogenes genome contains more than 20 genes that encode cell surface-associated proteins termed internalins, which are characterized by the presence of multiple leucine-rich repeats. Subsets of internalin genes have been reported previously as transcriptionally regulated by the stress responsive sigma factor B (inlA, inlB, inlC2 and inlD) and the pleiotropic transcriptional activator PrfA (inlA, inlB, inlC). To investigate contributions of Ï?B and PrfA to internalin gene regulation, we designed a sub-genomic microarray containing two probes for each of the 24 internalin-like genes present in the L. monocytogenes 10403S genome. Competitive microarray hybridization was performed on RNA extracted from (i) the 10403S parent strain and an isogenic Î?sigB strain; (ii) 10403S

ORGANISM(S): Listeria monocytogenes

SUBMITTER: Kathryn Boor 

PROVIDER: E-GEOD-6421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress