Genome-wide DNA-binding profile of the Vibrio cholerae histone-like nucleoid structuring protein (H-NS)

ABSTRACT: The cholera disease bacterium V. cholerae, can adopt planktonic or biofilm lifestyles depending on the intracellular concentration of the second messenger cyclic diguanylic acid (c-di-GMP). Biofilm formation protects Vibrios from stressful conditions and facilitates disease transmission by enhancing infectivity. The histone-like nucleoid structuring protein (H-NS) is a global regulator of genes associated with pathogenicity and responses to environmental stresses. H-NS represses the transcription of genes vpsT, vpsA and vpsL, which are required for the biosynthesis of the biofilm exopolysacchide matrix. Here we demonstrate that the c-di-GMP-binding protein VpsT disrupts H-NS nucleoprotein complexes at the vpsA and vpsL promoters and that this effect is enhanced by c-di-GMP. We used ChIP co

ORGANISM(S): Vibrio cholerae

SUBMITTER: Anisia Silva-Benitez 

PROVIDER: E-GEOD-64249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress