Peripheral regions and central regions from HT-29 tumors

ABSTRACT: Gene expression of peripheral regions and central regions from HT-29 tumors Sample tissues were immediately frozen by liquid nitrogen after isolation. Total RNAs were extracted from samples with a PureLink RNA Mini Kit (Life Technologies). The integrity of total RNAs was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Low Input Quick Amp Labeling Kit, one-color (Agilent Technologies) was used to prepare Cy3-labelled target cRNA according to the manufacturer's instructions. Labeled cRNAs were hybridized with a SurePrint G3 Human GE 8M-CM-^W60K Microarrays (Agilent Technologies). Three separate hybridizations were performed for each sample. Array images were captured using a DNA Microarray Scanner (Agilent Technologies), and data were analyzed using

ORGANISM(S): Homo sapiens

SUBMITTER: Yukie Yoshii 

PROVIDER: E-GEOD-64831 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress