Asymmetry of STAT action in driving IL-27 and IL-6 transcriptional outputs and cytokine specificity

ABSTRACT: IL-6 and IL-27 have antagonistic and overlapping functions, signal through a shared receptor subunit and employ the same downstream STAT proteins. To evaluate the degree of specificity and redundancy for these cytokines, we quantified global transcriptomic changes induced by the two cytokines and determined the relative contributions of STAT1 and STAT3 using genetic models and ChIP-seq. We found a high degree of overlap of the transcriptomes induced by IL-6 and IL-27 and extremely few examples in which the cytokines acted in opposition. Using STAT deficient cells and T cells from patients with gain-of-function STAT1 mutations, we show that STAT3 was responsible for the overall transcriptional output driven by both cytokines, whereas STAT1 was the driver of cytokine specificity. STAT1 did n

ORGANISM(S): Mus musculus

SUBMITTER: Yuka Kanno 

PROVIDER: E-GEOD-65621 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress