Frequent derepression of the mesenchymal transcription factor gene in acute myeloid leukemia (human)

ABSTRACT: Bone marrow samples from normal adult male donors were collected into EDTA. Red cells were removed by ammonium chloride lysis. Leukocytes were washed in SM buffer and CD34+ cells were separated from CD34- cells using an AutoMACS device and anti-CD34 immunomagnetic beads (Miltenyi Biotec), according to manufacturer’s instructions. For mature cell populations, CD34- cells were FACS purified according to the following immunophenotypes, with 7-AAD used to exclude dead cells: Neutrophils: side scatter high CD15+ CD16+. Monocytes: side scatter low-intermediate CD14+ CD16- CD15-. See also Huang et al., 2014. Eight exon arrays total; 4 arrays for each cell type; four separate individuals.

ORGANISM(S): Homo sapiens

SUBMITTER: Tim Somervaille 

PROVIDER: E-GEOD-66254 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress