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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data of E. histolytica trophozoites treated with 5AzaC

ABSTRACT: Expression profiling of treatment of E. histolytica HM-1:IMSS trophozoites with 5-Aza cytidine, an inhibitor of DNA methyltransferase Keywords: drug treatment HM1 trophozoites were treated for 3 or 7 days with 23 micromolar 5-Aza cytidine, and RNA extracted and analyzed on a custom Affymetrix array. Data from these two arrays is to be compared to expression data from HM1 trophozoites grown without drug exposure.

ORGANISM(S): Entamoeba histolytica

SUBMITTER: Gretchen Ehrenkaufer

PROVIDER: E-GEOD-6635 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression.

Ali Ibne Karim M IK Ehrenkaufer Gretchen M GM Hackney Jason A JA Singh Upinder U

BMC genomics 20070105

<h4>Background</h4>In higher eukaryotes DNA methylation regulates important biological functions including silencing of gene expression and protection from adverse effects of retrotransposons. In the protozoan parasite Entamoeba histolytica, a DNA methyltransferase has been identified and treatment with 5-azacytidine (5-AzaC), a potent inhibitor of DNA methyltransferase, has been reported to attenuate parasite virulence. However, the overall extent of DNA methylation and its subsequent effects o ...[more]

PMID: 17207281

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