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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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CDNA microarray analysis of splenic B220+CD138+ plasma cells from K/BxNsf and K/BxN mice

ABSTRACT: Long-lived plasma cells (LLPCs) develop under the help of follicular helper T (Tfh) cells and reside mainly in the bone marrow. However, these cells are unusually abundant in the spleen of several autoimmune models including K/BxNsf mice, yet their pathogenic impact remains unknown. To investigate a previously unappreciated role of splenic LLPCs, we sorted splenic plasma cells (PCs) from K/BxNsf and K/BxN mice, corresponding to LLPCs and conventional short-lived PCs, respectively, and compared their transcriptomes. The B220+CD138+ fraction from K/BxNsf and their control K/BxN mice were sorted by FACSariaIII.

ORGANISM(S): Mus musculus

SUBMITTER: Eunkyeong Jang

PROVIDER: E-GEOD-66947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Splenic Long-Lived Plasma Cells Promote the Development of Follicular Helper T Cells during Autoimmune Responses.

Jang Eunkyeong E Cho Wang Sik WS Oh Yeon-Kyung YK Cho Mi-La ML Kim Jung Mogg JM Paik Doo-Jin DJ Youn Jeehee J

Journal of immunology (Baltimore, Md. : 1950) 20160104 3

Long-lived plasma cells (LLPCs) develop under the help of follicular helper T (Tfh) cells and reside mainly in the bone marrow. However, these cells are unusually abundant in the spleen of several autoimmune models including K/BxNsf mice, yet their pathogenic impact remains unknown. To investigate a previously unappreciated role of splenic LLPCs, we sorted splenic plasma cells (PCs) from K/BxNsf and K/BxN mice, corresponding to LLPCs and conventional short-lived PCs, respectively, and compared t ...[more]

PMID: 26729802

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cDNA microarray analysis of splenic B220+CD138+ plasma cells from K/BxNsf and K/BxN mice

Project description:Long-lived plasma cells (LLPCs) develop under the help of follicular helper T (Tfh) cells and reside mainly in the bone marrow. However, these cells are unusually abundant in the spleen of several autoimmune models including K/BxNsf mice, yet their pathogenic impact remains unknown. To investigate a previously unappreciated role of splenic LLPCs, we sorted splenic plasma cells (PCs) from K/BxNsf and K/BxN mice, corresponding to LLPCs and conventional short-lived PCs, respectively, and compared their transcriptomes.

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| EGAS00001004623 | EGA

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