Tracing the formation of hematopoietic stem cells in mouse embryos by single-cell functional and RNA-Seq analyses [10-cell]

ABSTRACT: Hematopoietic stem cells (HSCs) in adult are specified early from the endothelium-derived precursors (e.g., hemogenic endothelium, and pre-HSCs) in mouse mid-gestation embryos, the detailed process, however, is still largely unknown due to their rareness, transience, and current inability to prospectively isolate them efficiently . Here we developed a potent set of surface markers that could capture the earliest emerging HSCs, the CD45- pre-HSCs with high accuracy and purity, as rigorously and functionally verified by single-cell-initiated serial transplantation assays. Then we applied single-cell RNA-Seq technique to analyze five populations related to HSC formation: the CD45- (type 1) and CD45+ (type 2) pre-HSCs as well as endothelial cells in the E11 AGM region; and later mature HSCs in

ORGANISM(S): Mus musculus

SUBMITTER: Xianlong Li 

PROVIDER: E-GEOD-66954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress