Mapping nucleosome resolution chromosome folding in yeast by Micro-C

ABSTRACT: We describe a Hi-C based method, Micro-C, in which micrococcal nuclease is used instead of restriction enzymes to fragment chromatin, enabling nucleosome resolution chromosome folding maps. Analysis of Micro-C maps for budding yeast reveals abundant self-associating domains similar to those reported in other species, but not previously observed in yeast. These structures, far shorted than topologically-associating domains in mammals, typically encompass one to five genes in yeast. Strong boundaries between self-associating domains occur at promoters of highly transcribed genes, and regions of rapid histone turnover that are typically bound by the RSC chromatin-remodeling complex. Investigation of chromosome folding in mutants confirms roles for RSC, “gene looping” factor Ssu72, Mediator, H

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Oliver Rando 

PROVIDER: E-GEOD-68016 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress