MRNA Sequencing of skeletal muscle genes in wildtype and BCATm (BCAT2) KO mice

ABSTRACT: Consumption of a protein containing meal by a fasted animal promotes protein accretion in skeletal muscle, in part through leucine stimulation of protein synthesis and indirectly through repression of protein degradation mediated by its metabolite, α-ketoisocaproate. Mice lacking the mitochondrial branched-chain aminotransferase (BCATm/Bcat2), that interconverts leucine and α-ketoisocaproate, exhibit elevated protein turnover. Here, the transcriptomes of gastrocnemius muscle from BCATm knockout (KO) and wildtype mice were compared using Next Generation RNA-Sequencing (RNA-Seq) to identify potential adaptations associated with their persistently altered nutrient signaling. Statistically significant changes in the abundance of 1486/~39,010 genes were identified. Bioinformatics analysis of

ORGANISM(S): Mus musculus

SUBMITTER: Yuka Imamura Kawasawa 

PROVIDER: E-GEOD-68915 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress