Treatment effects of trastuzumab and TDM-1 in a murine model of breast cancer brain metastasis

ABSTRACT: Background: Central nervous system (CNS) metastases represent a major problem in the treatment of HER2-positive breast cancer due to the disappointing efficacy of HER2-targeted therapies in the brain microenvironment. The antibody-drug conjugate ado-trastuzumab emtansine (T-DM1) has shown efficacy in trastuzumab-resistant systemic breast cancer. Here, we tested the hypothesis that T-DM1 could overcome trastuzumab resistance in preclinical models of brain metastases. Methods: We treated mice bearing BT474 or MDA-MB-361 tumors in the CNS (N=9-11 per group), or cancer cells grown in organotypic brain slice cultures with trastuzumab or T-DM1 at equivalent or equipotent doses. Using intravital imaging, molecular techniques and histological analysis we determined tumor growth, mouse survival, c

ORGANISM(S): Homo sapiens

SUBMITTER: Kamila Naxerova 

PROVIDER: E-GEOD-69042 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress