Conversion of gastric epithelia to endoderm progenitors by small molecules [DNA methylation]

ABSTRACT: Forced expression of transcription factors for lineage reprogramming brings hope to cell-based therapy. However, its application is hampered by risks of potential genetic aberrations and tumorigenicity. Using defined small molecules in presence of gastric stromal cells as feeders, we reprogramed human gastric epithelia into induced multipotent endodermal progenitors (hiMEPs) at efficiency of up-to-6%. The hiMEPs expressed genes relative to endodermal lineages but not associating with pluripotency, and could be expanded clonogenically remaining undifferentiation. Upon induction, hiMEPs were able to give rise to multiple functional endodermal cell types, apart from ectodermal or mesodermal lineages. TGFβ inhibition and particular Wnt signaling activation were crucial in reprogramming proces

ORGANISM(S): Homo sapiens

SUBMITTER: Jinhua Qin 

PROVIDER: E-GEOD-69705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress