Conversion of Human Somatic Cells to Multipotent Endoderm Stem Cells by Small Molecules [array]

ABSTRACT: Forced expression of transcription factors for lineage reprogramming brings hope to cell-based therapy. However, its application is hampered by risks of potential genetic aberrations and tumorigenicity. Using defined small molecules in presence of gastric stromal cells as feeders, we reprogramed human gastric epithelia into induced multipotent endodermal progenitors (hiMEPs) with efficiency of up-to-6%. The hiMEPs expressed genes relative to endodermal lineages but not associating with pluripotency, and could be expanded clonogenically remaining as undifferentiated colonies. Upon induction, hiMEPs were able to give rise to multiple functional endodermal cell types, apart from ectodermal or mesodermal lineages. TGFβ inhibition and particular Wnt signaling activation were crucial in reprogr

ORGANISM(S): Homo sapiens

SUBMITTER: Jinhua Qin 

PROVIDER: E-GEOD-71301 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress