Dynamics of gene expression changes in epithelial and mesenchymal HMLER cell lines transitioning from suspension 3D culture to adhesion cultures

ABSTRACT: In order to metastasize, few cells need to detach from the primary tumor, and transition through the circulation to distant sites to establish new solid tumor metastases. The gene expression changes occurring between these transitions are poorly understood, and controversial findings have been reported regarding transitions between epithelial (E) and mesenchymal (M) cell states. To model the metastasis process in vitro, here we examined the dynamics of gene expression changes and respective cell fates that are occurring in cells that are transitioning from adhesion culture to suspension 3D culture (mammospheres) and back to adhesion culture in vitro. We found that mammosphere and replating cultures of epithelial (E) cell lines (HP cells and E clones) led to enrichment for mesenchymal (M)

ORGANISM(S): Homo sapiens

SUBMITTER: Anne Grosse-Wilde 

PROVIDER: E-GEOD-70279 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress