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Orbitrap proteomic analysis of five distinct molecular subtypes of High Grade Serous Ovarian Carcinoma (HGSOC) under serum deprived and serum fed conditions

ABSTRACT: Cell lines representing five distinct phenotypes of high-grade serous ovarian carcinoma (HGSOC), including Epithelial (E), Intermediate-Epithelial (iE), Epithelial-Mesenchymal Hybrid (E/M), Intermediate-Mesenchymal (iM), and Mesenchymal (M), previously classified in our laboratory based on the expression patterns of the transcription factors TCF21 and SLUG (Varankar. S. et al. Carcinogenesis. 2019), were subjected to 48 hours of serum starvation (SS) or maintained in normal serum-fed conditions (+S) 24 hours post-seeding in triplicate. Following the treatment conditions, samples were harvested and analyzed using Orbitrap mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Aravindan Narayanan

LAB HEAD: Sharmila A.

PROVIDER: PXD062888 | Pride | 2025-10-08

REPOSITORIES: Pride

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			Action	DRS
	A4_SEARCHfile.zip	Other
	CaOV3_SEARCHfile.zip	Other
	OVCA420_SEARCHfile.zip	Other
	OVCAR3_SEARCHfile.zip	Other
	OVCAR3proteinGroups.txt	Txt

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Publications

Targeting mitochondrial translation and OXPHOS in high-grade serous ovarian carcinoma eliminates stem-like cells.

Narayanan Aravindan A Guha Souvik S Mali Avinash A Bapat Sharmila A SA

Cell death & disease 20251006 1

Ex vivo stem cell self-renewal and maintenance is supported by absence of serum-derived mitogens. In the present study, we sought to elucidate the proteomes of stem-like cells grown in serum-free media across a panel of high-grade serous ovarian cancer cell lines, which encompass a gradient from epithelial, intermediate and mesenchymal cell phenotypes to recapitulate the heterogeneity of the disease. MaxQuant-based label-free quantification of proteins identified that despite their different cel ...[more]

PMID: 41053051

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microRNA profiling of serum microRNA from high grade serous ovarian carcinoma

Project description:High-grade serous ovarian carcinoma (HGSOC) is the most lethal gynecologic neoplasm, with five-year survival rate below 30%. Early disease detection is of utmost importance to improve the cure rate of HGSOC. Liquid biopsies are now becoming a new paradigm to develop novel biomarkers with diagnostic and prognostic purposes. The focus of this study was to detect the levels of circulating miRNAs in tissues and sera from patients with HGSOC and to evaluate their diagnostic value. To this end, an array-based discovery platform, followed by an innovative statistical approach of data normalization, was exploited, to identify miRNA species selectively expressed in serum of patients with HGSOC. Sera from 106 high grade serous ovarian carcinoma (HGSOC) and 24 healthy controls were used for profiling serum microRNA using a modified version of a commercially available microarray, with the aim of identifying differentially expressed microRNA between tumor patients and healthy controls.

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Slug-induced miR-137 expression contributes to non-small cell lung cancer progression

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Integrative proteomic profiling of ovarian cancer cell lines reveals precursor-cell associated proteins and functional status

Project description:A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumor of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumors, immortalized ovarian surface epithelial cells, and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantitation of > 10,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II), and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic dataset, as well as a confirmatory publicly available CPTAC/TCGA tumor proteome dataset, into a predominantly epithelial and mesenchymal HGSOC tumor cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumors indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium.

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Distinct EMT programs control normal stem cells and cancer stem cells of mammary tissue

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Acutely Injured Kidneys Release Proteins into the Serum that Cause Oxidative Stress

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2017-03-16 | GSE94678 | GEO

The invasion promoter Slug is a critical cell cycle regulator

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The invasion promoter Slug is a critical cell cycle regulator

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