Negative-Pressure Induces p120-Catenin Dependent Adherens Junction Disassembly

ABSTRACT: Background: Negative-pressure wound therapy has become widely available in modern chronic wound cares. Accelerated keratinocyte (HaCaT cell) movements and decreased E-cadherin expressions induced by the applied negative pressure gradient of 125 mmHg (NP) have been reported in previous studies. However, the molecular mechanism for E-cadherin regulations under NP remains unexplored. We highlighted the signal transduction involved in NP-induced E-cadherin regulation for keratinocytes in the study. Results: Microarray results showed that catenin 1 (CTNND1) gene, the gene encoding the p120-catenin (p120ctn) in cell-cell junctions, was significantly (p = 0.0005) upregulated in HaCaT cells under NP for 12 hours in comparison with those at ambient pressure (AP). Cell fractionations and immunoblott

ORGANISM(S): Homo sapiens

SUBMITTER: Yun-Shien Lee 

PROVIDER: E-GEOD-70512 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress