Comparative analyses of CTCF and BORIS occupancies uncover two distinct classes of CTCF binding genomic regions

ABSTRACT: CTCF and BORIS (CTCFL), two paralogous mammalian proteins sharing nearly identical DNA binding domains, are thought to function in mutually exclusive manners in DNA binding and transcriptional regulation. Here we show that these two proteins co-occupy a specific subset of regulatory elements consisting of clustered CTCF binding motifs (termed 2xCTSes). BORIS occupancy at 2xCTSes is largely invariant in BORIS-positive cancer cells, with the genomic pattern recapitulating the germline-specific BORIS binding to chromatin. In contrast to the single-motif CTCF target sites (1xCTSes), the 2xCTS elements are preferentially found at active promoters and enhancers, both in cancer and germ cells. 2xCTSes are also enriched in genomic regions that escape histone to protamine replacement in human and m

ORGANISM(S): Homo sapiens

SUBMITTER: Elena Pugacheva 

PROVIDER: E-GEOD-70764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress