Orientation-Specific Joining of AID-initiated DNA Breaks Promotes Antibody Class Switching

ABSTRACT: During B cell development, RAG endonuclease cleaves immunoglobulin heavy chain (IgH) V, D, and J gene segments and orchestrates their fusion as deletional events that assemble a V(D)J exon in the same transcriptional orientation as adjacent Cμ constant region exons. In mice, six additional sets of constant region exons (CHs) lie 100-200kb downstream in the same transcriptional orientation as V(D)J and Cμ exons. Long repetitive switch (S) regions precede Cμ and downstream CHs. In mature B cells, class switch recombination (CSR) generates different antibody classes by replacing Cμ with a downstream CH. Activation Induced Cytidine Deaminase (AID) initiates CSR by promoting deamination lesions within Sμ and a downstream acceptor S region; these lesions are converted into DNA double strand brea

ORGANISM(S): Mus musculus

SUBMITTER: Junchao Dong 

PROVIDER: E-GEOD-71005 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress