Transcription profiling of human samples to identify markers for early diagnosis of of endoprosthesis loosening

ABSTRACT: The aim of the study was to identify markers for the early diagnosis of endoprosthesis loosening, for the differentiation between wear-particle induced and septic loosening, as well as to gather new insights into the pathogenesis. 824 genes were differentially expressed with a fold change greater than 2. Among these were Chitinase 1, CD52, Calpain 3, Apolipoprotein, CD18, Lysyl oxidase, Cathepsin D, E-Cadherin, VE-Cadherin, Nidogen, Angiopoietin 1 and Thrombospondin 2, and the differential expression levels were validated by RT-PCR. The chitinase activity was significantly higher in the blood from patients with wear-particle induced prosthesis loosening (p=0.001). However, using chitinase activity as a marker for early diagnosis, it has a specificity of 83% and a sensitivity of only 52%, d

ORGANISM(S): Homo sapiens

SUBMITTER: Lars Morawietz 

PROVIDER: E-GEOD-7103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress