ADAR1-mediated A-to-I RNA editing is essential for erythropoiesis [RNA-seq]

ABSTRACT: Purpose: RNA editing by ADAR1 is essential for hematopoietic development. The goals of this study were firstly, to identify ADAR1-specific RNA-editing sites by indentifying A-to-I (G) RNA editing sites in wild type mice that were not edited or reduced in editing frequency in ADAR1 deficient murine erythroid cells. Secondly, to determine the transcription consequence of an absence of ADAR1-mediated A-to-I editing. Methods: Total RNA from E14.5 fetal liver of embryos with an erythroid restricted deletion of ADAR1 (KO) and littermate controls (WT), in duplicate. cDNA libraries were prepared and RNA sequenced using Illumina HiSeq2000. The sequence reads that passed quality filters were analyzed at the transcript level with TopHat followed by Cufflinks. qRT–PCR validation was performed using

ORGANISM(S): Mus musculus

SUBMITTER: Alistair Chalk 

PROVIDER: E-GEOD-71042 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress