Evolutionary and ontogenetic changes in RNA editing in human, chimpanzee and macaque brains

ABSTRACT: we used RNA-Seq to quantify the RNA editing level at more than 8,000 previously annotated exonic A-to-I RNA editing sites in two brain regions - prefrontal cortex and cerebellum - of humans, chimpanzees and rhesus macaques. We observed substantial conservation of RNA editing levels between the brain regions, as well as among the three primate species. Evolutionary changes in RNA editing were nonetheless evident among the species. Across lifespan, we observed an increase of the RNA editing level with advanced age in both brain regions of all three primate species. poly(A) enriched RNAs extracted from pooled samples of two brain regions: CBC and PFC of chimpanzee and macaque, fragmented, revers transcribed to double-stranded cDNA using random hexamers. Sequencing libraries were prepared acc

ORGANISM(S): Macaca mulatta

SUBMITTER: zs li 

PROVIDER: E-GEOD-45244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress