Density Gradients Separate Dormant and Treatment-Resistant Human Glioblastoma Cells

ABSTRACT: Glioblastoma (GBM) is among the most aggressive cancers. Despite aggressive radiotherapy and treatment with the alkylating agent temozolomide (TMZ), patients ultimately succumb to the disease. Although much interest has focused on highly tumorigenic GBM stem cells (GSCs), adaption of a concept from microbial research proposes that a minor population of dormant “persister” cells in cancer evade current therapies. To separate dormant and treatment-resistant tumor cells in human GBM tumorspheres, we have refined density gradient protocols previously used for separation of neurosphere-forming neural stem cells (NSCs). We find that a minor cell population in human GBM tumorsphere cultures and patient-derived tumor biopsies display increased cell density. These high-density GBM cells (HDGCs)

ORGANISM(S): Homo sapiens

SUBMITTER: David Quigley 

PROVIDER: E-GEOD-71116 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress