Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Using the combined analysis of transcripts and metabolites to propose key genes for differential terpene accumulation across two regions

ABSTRACT: To obtain an interpretation from the view of transcriptome on distinct metabolite accumulation between ecologically different regions in China, next-generation sequencing technology was performed on E-L 31, 35?36 and 38 stages of Muscat Blanc a Petits Grains grape berries from Changli (CL, eastern) and Gaotai (GT, western). Transcriptome analysis revealed that some key genes involved in terpene synthesis were markedly up-regulated in the CL region. Particularly in the MEP pathway, the expression of VvHDR (1-hydroxy-2-methyl-2-butenyl 4-diphosphate reductase) paralleled with the accumulation of terpenes, which can promote the flow of isopentenyl diphosphate (IPP) into the terpene synthetic pathway. The glycosidically bound monoterpenes accumulated differentially along with maturation in both regions, which is synchronous with the expression of a monoterpene glucosyltransferase gene (VvGT14). Other genes were also found to be related to the differential accumulation of terpenes and monoterpene glycosides in the grapes between regions. Transcription factors that could regulate terpene synthesis were predicted through gene co-expression network analysis. Additionally, the genes involved in abscisic acid (ABA) and ethylene signal responses were expressed at high levels earlier in GT grapes than in CL grapes. cDNA libraries generated from four developmental stages (E-L 31, 35, 36 and 38) of Muscat Blanc a Petits Grains grape berries in two consecutive years from Changli (CL) and Gaotai (GT) in China were sequenced using Illumina HiSeq™ 2000.Only one RNA-seq library was constructed for each of the E-L31 and E-L36 stage samples because of the small amount of high quality RNA acquired, whereas two libraries were constructed for each of the E-L35 and E-L38 stage samples. As a result, a total of 24 libraries were obtained for the two regions within two years.

ORGANISM(S): Vitis vinifera

SUBMITTER: Qiu-hong Pan

PROVIDER: E-GEOD-71146 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Using the combined analysis of transcripts and metabolites to propose key genes for differential terpene accumulation across two regions.

Wen Ya-Qin YQ Zhong Gan-Yuan GY Gao Yuan Y Lan Yi-Bin YB Duan Chang-Qing CQ Pan Qiu-Hong QH

BMC plant biology 20151006

<h4>Background</h4>Terpenes are of great interest to winemakers because of their extremely low perception thresholds and pleasant floral odors. Even for the same variety, terpene profile can be substantially different for grapevine growing environments. Recently a series of genes required for terpene biosynthesis were biochemically characterized in grape berries. However, the genes that dominate the differential terpene accumulation of grape berries between regions have yet to be identified.<h4> ...[more]

PMID: 26444528

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Using the combined analysis of transcripts and metabolites to propose key genes for differential terpene accumulation across two regions

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Using the combined analysis of transcripts and metabolites to propose key genes for differential terpene accumulation across two regions.

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