Sulphur dioxide evokes large scale reprogramming of the grape berry transcriptome
Ontology highlight
ABSTRACT:
however there is increasing concern as to the health rises associated with human consumption.
Thus, we investigated the transcript abundance changes in grapes treated with sulfur dioxide and
other preservative compounds, compared to control treatments. Export quality, red-skinned M-^QCrimson
SeedlessM-^R grapes (Vitis vinifera L.) were harvested at commercial maturity from one vineyard
in the Swan Valley region of Western Australia. At least 15 kg grapes per treatment were
completely immersed in the treatment solution (? 1 min) and allowed to dry on racks before
being weighed in to 7 x 2 kg lined export cartons, without sulphur pads. Once packed, cartons
were immediately placed in 2 M-0C storage for up to 56 days and once cool, commercial
SO2-generating pads were placed into cartons for SO2 treatment. The salicylic acid (SA,
25 mM), methyl jasmonate (MJ, 5mM) and their combination (25 mM SA + 5 mM MJ) were
dissolved in 0.5 % (v/v) dimethyl sulphoxide (DMSO). A 0.5 % solution of DMSO was used
as a control treatment. An additional, untreated control for sulphur-treated berries was used.
Microarrays were performed in duplicate for the 6 treatments, Sulfur dioxide (plus untreated
control), SA, MJ, and the combined SAMJ treatment (plus DMSO control) on grape berries
after 21 days of treatment post harvest. The results indcate a large scale re-programing of
the grape berry transcritpome, similar to that which has been observed for prolonged
exposure to harsh oxidative stress conditions.
ORGANISM(S): Vitis vinifera
SUBMITTER: Estelle Giraud
PROVIDER: E-MEXP-3045 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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