Transcription profiling of grapevine cell culture treated with methyly jasmonate, salicylic acids and ethanol controls

ABSTRACT: Calli generated from grape berries were used to establish a cell suspension in Gamborgs B5 liquid medium with minimal organics supplemented with 30 gL-1 sucrose, 0.25 gL-1 casein hydrolysate, 0.93 ?M kinetin and 0.54 ?M naphthaleneacetic acid (NAA) and incubated in darkness at 26°C.
The cells were cultured in 250 mL flasks (50 mL of cell suspension in each) incubated in darkness on an orbital shaker at 100 rpm. The cell suspensions were sub-cultured every 2 weeks using an initial packed cell volume (PCV) of 15%. For the evaluation of the effect of elicitor addition on the changes in gene expression, 100 mL flasks containing 20 mL of the above cell suspension were used. Each treatment was conducted in triplicate.

In a set of preliminary experiments studying the effect of elicit

ORGANISM(S): Vitis vinifera

SUBMITTER: Paul Boss 

PROVIDER: E-MEXP-1524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress