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Trichoderma reesei QM6a SNI/BNI degradation experiment

ABSTRACT: Goal :
Finding of bio-degradation products of several native SNIs and BNIs in Trichoderma reesei QM6a liquid minimal medium (12C and 13C) cultures.

Minimal medium
Amount for 500 mL medium | Actual | amount Substance
1.7500 g | 1.75115 g | (NH4)2SO4
2.5000 g | 2.51394 g | KH2PO4
0.6200 g | 0.63039 g | MgSO4 . 7 H2O
0.3125 g | 0.20447 g | NaCl
10 mL | 2 x 5 mL | 50X Trace element solution
ad 490 mL | 490 mL | autoclaved ELGA water
plus either 0.25g D-Glucose-13C or 0.25g D-Glucose-12C

Tested BNIs/SNIs (1.50mg each in D2O)
3,4-Dimethyl-1H-pyrazole phosphate (DMPP) [SOS 896]
Methyl p-coumerate (MHPA) [no SOS]
2-Amino-4-chloro-6-methylpyrimidine (ACMP) [no SOS]
Coixol (MBOA) [no SOS]
Karanjin
Sakuranetin
Syringic acid (SA) [SOS 899]
3,4-Dihydroxybenzaldehyde (DHBA) [SOS 900]
(R)-(+)-Limonene [SOS 277]

Trichoderma reesei QM6a spores on two MEX plates,
incubated with QM6a on 2024-08-22 (four days).
Transfer to BOKU IFA-Tulln.
Continued incubation at 28C, 90% relative humidity in constant darkness.

Inoculation and pre-cultivation:
Weighted glucose and filled up to respective volume with prepared minimal medium without carbon source
Sterile filtered prepare medium (at Lamina)
Inoculated medium with 15 uL spore solution per well (12C glucose) or 7 uL spore solution per well (U-13C6 glucose) respectively
Incubation at 28C, 70 % relative humidity in constant darkness in Memmert HPP260 Incubator

Treatment of cultures with SNI and BNI solutions and addition of more glucose

Sampling after 7 days

Measurements according to 10.1021/ac503290j, but with an Q Exactive HF instrument

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Trichoderma Reesei Qm6a (ncbitaxon:431241)

SUBMITTER: christoph bueschl

PROVIDER: MSV000100562 | MassIVE | Fri Jan 23 01:46:00 GMT 2026

REPOSITORIES: MassIVE

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