Transcription profiling of human tumor-conditioned versus quiescent HUVEC cells, and 5-aza-2a??-deoxycytidine and trichostatin AI treated cells to identify epigenetically silenced genes

ABSTRACT: Tumor angiogenesis requires intricate regulation of gene expression in endothelial cells (EC). We recently showed that DNA methyltransferase (DNMT)- and histone deacetylase (HDAC) inhibitors directly repress EC growth and tumor angiogenesis, suggesting that epigenetic modifications mediated by DNMTs and HDACs are involved in regulation of EC gene expression during tumor angiogenesis. To understand the mechanisms behind the epigenetic regulation of tumor angiogenesis, we used microarray analysis to perform a comprehensive screen to identify genes downregulated in tumor-conditioned versus quiescent EC, and re-expressed by 5-aza-2’-deoxycytidine and trichostatin A. Among the 81 genes identified, 77% harboured a promoter CpG island. Validation of mRNA levels of a subset of genes confirmed si

ORGANISM(S): Homo sapiens

SUBMITTER: Debby Hellebrekers 

PROVIDER: E-GEOD-7132 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress