Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data in CD4 T cells from lungs of PBS or house dust mite treated mice

ABSTRACT: CD4 T cells are essential mediators of the asthmatic process. We used the clinically relevant allergen house dust mites to induce signs of allergy in mice and performed gene expression arrays specifically on CD4 T cells infiltrating the lung Reference: IL-21-producing CD4+ T cells promote type 2 immunity to house dust mites Primary CD4+ T cells were isolated from mice sensitised and challenged to either house dust mites or PBS. Purification of CD4 T cells was performed by flow cytometry. RNA was isolated, converted to cDNA and then hybridised on Affymetrix GeneChip Mouse Gene 2.0 ST Arrays

ORGANISM(S): Mus musculus

SUBMITTER: Bart Lambrecht

PROVIDER: E-GEOD-72005 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

Expression data in CD4 T cells from lungs of PBS or house dust mite treated mice

Project description:CD4 T cells are essential mediators of the asthmatic process. We used the clinically relevant allergen house dust mites to induce signs of allergy in mice and performed gene expression arrays specifically on CD4 T cells infiltrating the lung Reference: IL-21-producing CD4+ T cells promote type 2 immunity to house dust mites

2015-08-13 | GSE72005 | GEO

scRNA-seq analysis of lung neutrophils from mice, 24 hours after intranasal exposure to vehicle, 100 ng LPS and 10 µg LPS

Project description:Mice intranasally exposed to a low dose of LPS (i.e., 100 ng) are prone to develop features of allergic asthma upon subsequent exposure to house dust mites (HDM) allergens, while mice exposed to vehicle or 100 µg LPS do not develop such features. In order to understand the mechanisms that promote allergic asthma, we sought to characterize the lung neutrophils, which are massively recruited after LPS exposure, by single cell RNA-Seq.

2019-07-25 | E-MTAB-6902 | biostudies-arrayexpress

Early Activation of Th2 Inflammatory and Pruritogenic Pathways in Acute Canine Atopic Dermatitis Skin Lesions

Project description:To characterize the inflammatory events occuring during early experimental acute AD lesions, skin biopsies were collected 6, 24, and 48 h after epicutaneous application of Dermatophagoides farinae house dust mites (HDM) to sensitized atopic dogs.

2015-11-02 | GSE74136 | GEO

Study of Foxp3 expression in tumor-associated macrophages

Project description:Using 5 differents approaches, including RNA sequencing, we demonstrated that macrophages that specifically infiltrate renal tumors, express the immunosuppressive transcription factor Foxp3. Examination of the Foxp3 mRNA expression in 3 different cell subsets (including CD4 T cells (CD4), type-1 macrophages (M1) and type-2 macrophages (M2))

2014-05-31 | E-GEOD-56904 | biostudies-arrayexpress

Gene expression patterns in house dust mite stimulated CD4 T cells and IgG:IgE ratios

Project description:PBMC from house dust mite (HDM) sensitized atopics were cultured in the presence or absence of HDM extract for 24 hours. At the termination of the cultures, CD4 T cells were isolated using immunomagnetic separation. Gene expression was profiled on microarrays. The study design consisted of 45 subjects and two conditions (medium control, HDM stimulation).

2015-11-03 | E-GEOD-70760 | biostudies-arrayexpress

Gene expression patterns in allergen-driven CD4 T cell responses from human atopics with or without asthma.

Project description:PBMC from house dust mite (HDM) sensitized atopics with or without asthma (or nonallergic controls) were cultured in the presence or absence of HDM extract for 24 hours. At the termination of the cultures, immunomagnetic separation was performed to purify CD4 T cells. Gene expression was profiled on microarrays. The study design consisted of 72 subjects, and two experimental conditions (medium control, HDM stimulation).

2016-02-25 | E-GEOD-73482 | biostudies-arrayexpress

Proteomic analysis of Blomia tropicalis bodies and feces

Project description:This dataset contains nano-LC-MS/MS analyzed samples of the domestic (house dust) mite Blomia tropicalis, which is a major source of allergens in tropical and subtropical regions. For proteomic analyses, four sample types, each with three biological replicates, were prepared: (i) 1000 individually collected adult mites, (ii) pools of mites of different ages, including eggs; (iii) water extracts of feces, and (iv) detergent-buffer extracts of the remaining pellet of the water extract. Laboratory culture of B. tropicalis was maintained as described previously at the Crop Research Institute, Prague, Czechia. Mites were mass reared in tissue culture flasks on a house dust mite diet (HDMd) in a large population (approximately 4 weeks) when the diet is almost consumed. This study was supported by LUAUS23082 of the Czech Ministry of Education, Youth and Sports.

2023-05-04 | MSV000091854 | MassIVE

Gene expression profile of Foxp3 expressing naturally ocurring regulatory macrophages from mice

Project description:Define the genetic profile of naturally occuring regulatory macrophages that express Foxp3 (MacRegs) compared to Foxp3 neg macrophages, to determine candidate genes responsible for their regulatory function. Compare this new cellular population with Tregulatory cells. Two conditions were compared: Fresh CD11b+ F4/80+ FOXP3+ cells (3 independent isolates) and CD11b+ F4/80+ FOXP3- cells (3 independent isolates). MacRegs were also compared to CD4+Foxp3+ Tregs .

2011-04-01 | E-GEOD-23793 | biostudies-arrayexpress

Infiltrating monocyte-derived macrophages and resident kupffer cells display different ontogeny and functions in acute liver injury

Project description:Molecular profiling of infiltrating monocyte-derived macrophages versus resident kupffer cells following acute liver injury The liver has a remarkable capacity to regenerate after injury; yet, the role of macrophages (MF) in this process remains controversial mainly due to difficulties in distinguishing between different MF-subsets. Here, we utilized a murine model of acute liver injury caused by overdose of acetaminophen (APAP) and defined three distinct MF subsets that populate the liver following injury. Accordingly, resident Kupffer cells (KC) were significantly reduced upon APAP-challenge and started recovering by self-renewal at resolution phase without contribution of circulating Ly6Chi monocytes. The latter were recruited in a CCR2 and M-CSF mediated pathway at the necro-inflammatory phase and differentiated into ephemeral Ly6Clo MF subset at resolution phase. Moreover, their inducible ablation resulted in impaired recovery. Microarray based molecular profiling uncovered high similarity between steady state KC and those recovered at the resolution phase. In contrast, KC and monocyte-derived MF displayed distinct pro-restorative genetic signature at the resolution phase. Finally, we show that infiltrating monocytes acquire a pro-restorative polarization manifested by unique expression of pro-angiogenesis mediators and genes involved with inhibition of neutrophil activity and recruitment and promotion of their clearance. Collectively, our results present a novel phenotypic, ontogenic and molecular definition of liver-MF compartment following acute injury. 11 Samples (arrays) were performed. We generated pairwise comparison between all the different macrophages stages, using Partek Genomics Suite. Genes with p?5%[FDR] and a fold-change difference of ?2 or <-2 were selected.

2014-08-01 | E-GEOD-55606 | biostudies-arrayexpress

RORγt -expressing FOXP3+ skin-resident Treg cells derived from the periphery in Allergen-Specific Immunotherapy

Project description:The study explored the impact of Specific Immunotherapy on patients with Atopic Dermatitis sensitized to house dust mites by analyzing the transcriptomes of T regulatory cells before and after treatment. Result found that effective SIT is associated with distinct gene expression changes in Treg cells, particularly increased expression of Treg markers and RORC, a gene linked to immune regulation. These results indicate that successful SIT may enhance the function of Treg cells expressed RORC and help restore immune balance in these patients.

2024-05-01 | GSE252205 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data