HT-SuperSAGE of the gut tissue of a Vip3A-resistant Heliothis virescens F. (Lepidoptera: Noctuidae) strain provides insights into its evolution and toxin adaptation

ABSTRACT: Summary: Multi-toxins Bt-crops carrying insecticidal toxins with similar host spectrum and different mode of action e.g. Cry and Vip, are expected to improve resistance management in target pests. Control failure has been informed for Cry toxins but not for Vip3A, of which no mechanism of resistance has yet been identified. Here we applied HT-SuperSAGE to analyze the transcriptome profiling in the midgut tissue of a tobacco budworm Heliothis virescens (F.) strain laboratory-selected for Vip3A resistance. A total of 1324252 26-bp tags were sequenced representing 17751 unique transcripts (UniTags) from genetically similar Vip3A-resistant (Vip-Sel) strain and susceptible control (Vip-Unsel) strain. Differential expression was found significant (≥ 2.5-fold or ≤ 0.4) for 1845 unigenes that cons

ORGANISM(S): Heliothis virescens

SUBMITTER: Camilo Ayra-Pardo 

PROVIDER: E-GEOD-72228 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress