AP-1 family members act at DNA targets in conjunction with Sox9 to promote chondrocyte hypertrophy

ABSTRACT: A comprehensive analysis of Sox9 binding profiles in developing chondrocytes identified marked enrichment of an AP-1-like motif (Ohba et al. 2015). Here, we have explored the functional interplay between Sox9 and AP-1 in mammalian chondrocyte development. Among AP-1 family members, Jun and Fosl2 were highly expressed within prehypertrophic and early hypertrophic chondrocytes. Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) showed a striking overlap in Jun- and Sox9-bound regions throughout the chondrocyte genome, a reflection of direct binding of each factor to target motifs in shared enhancers, and physical interactions of AP-1 with Sox9. In vitro expression analysis indicates that direct co-binding of Sox9 and AP-1 at target motifs enhanced target gene expression, whi

ORGANISM(S): Mus musculus

SUBMITTER: Xinjun He 

PROVIDER: E-GEOD-73372 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress