Transcription profiling of human G-CSF-mobilized Peripheral Blood Stem Cell Allograft

ABSTRACT: Granulocyte-colony stimulating factor (G-CSF) is used to boost granulocyte counts in immunocompromised patients, but its effects on the immune system may be counter productive. We tested the hypothesis that G-CSF mobilized peripheral blood stem cell (PBSC) products are immunologically down regulated based on gene microarray analysis. Ten peripheral blood samples from normal donors for allogeneic PBSC transplantation were obtained before and after administration of G-CSF and tested on Affymetrix Human U133 Plus 2.0 GeneChip® microarrays. Significant changes in gene expression after G-CSF mobilization were reported by controlling the false discovery rate at 5%. Immune-related genes were isolated from the data set and categorized according to probe set annotations and a thorough, independent

ORGANISM(S): Homo sapiens

SUBMITTER: Matthew Buzzeo 

PROVIDER: E-GEOD-7400 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress