Modification of T cell responses by stem cell mobilization requires direct signalling of the T cell by G-CSF and IL-10

ABSTRACT: The majority of allogeneic stem cell transplants are currently undertaken using G-CSF mobilized peripheral blood stem cells. G-CSF has diverse biological effects on a broad range of cells and IL-10 is a key regulator of many of these effects. Using mixed radiation chimeras in which the haematopoietic or non-haematopoietic compartments were wild-type (WT), IL-10–/–, G-CSFR–/– or combinations thereof we demonstrated that the attenuation of alloreactive T cell responses after with G-CSF mobilization required direct signalling of the T cell by both G-CSF and IL-10. IL-10 was generated principally by radio-resistant tissue, and was not required to be produced by T cells. G-CSF mobilization significantly modulated the transcription profile of CD4+CD25+ regulatory T cells, promoted their expansio

ORGANISM(S): Mus musculus

SUBMITTER: Glen Boyle 

PROVIDER: E-GEOD-54616 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress