The dynamic interactome and genomic targets of Polycomb complexes during stem cell differentiation

ABSTRACT: While the core members of the Polycomb family of proteins (PRC2, PRC1, PR-DUB) are well-characterized, little is known about the specific composition of and protein-protein interactions within these complexes in different cell types. We performed quantitative interaction proteomics and cross-linking mass spectrometry on core Polycomb complex members to identify novel interactors, the relative abundance (stoichiometry) of subunits, and the architecture of these complexes in mouse embryonic stem cells (mESCs) and neural progenitor cells (NPCs). Differentiation to NPCs resulted in dramatic binding changes for several substoichiometric interactors of PRC2 and PRC1. ChIP-seq of core PRC2 and PRC1 subunits in mESCs and NPCs also identified dynamic changes in the genomic localization of these c

ORGANISM(S): Mus musculus

SUBMITTER: Susan Kloet 

PROVIDER: E-GEOD-74330 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress