Cotranslational signal independent SRP preloading during membrane targeting

ABSTRACT: Cotranslational targeting into the endoplasmic reticulum (ER) by the Signal Recognition Particle (SRP) is a key event determining polypeptide fate in eukaryotic cells. Here, we globally define the principles and mechanisms of SRP binding and ER targeting in vivo. Cotranslational targeting through SRP is the dominant route into the ER for all secretory proteins, regardless of targeting signal characteristics. Cytosolic SRP functions in a pioneer translation round that builds a membrane-resident mRNAs pool, explaining how low SRP levels suffice for the secretory load. SRP does not induce an elongation arrest; consequently, kinetic competition between targeting and translation elongation dictates which substrates are translocated post-translationally. Unexpectedly, SRP binds most secretory ri

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Justin Chartron 

PROVIDER: E-GEOD-74393 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress