Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Aging reduces the pro-tumorigenic potential of bone marrow cells and influences triple-negative breast cancer progression

ABSTRACT: To examine the effects of recombinant granulin on human mammary stromal fibroblasts, we cultured immortalized GFP+ normal human mammary fibroblasts in the presence of recombinant human granulin (1ug/ml) or PBS every 24h for 6 days. To generate GRN-independent CAFs, we injected immortalized GFP+ human mammary fibroblasts, MCF7Ras human breast carcinoma cells, and 20% Matrigel subcutaneously into nude mice. Tumors were allowed to form for a period of 45 days. GFP+ fibroblasts were isolated from tumors by mincing the tumors, dissociating, and culturing in the presence of 1 ug/ml puromycin for ~3-4 weeks. CAF purity was confirmed by ensuring that 100% of the population was GFP-positive. We cultured normal human mammary fibroblasts in the presence human granulin (1ug/ml) or PBS control every 24h for 6 days. The analysis showen are for 3 control (control) and 3 granulin-treated (PGRN) fibrobast cultures.

ORGANISM(S): Homo sapiens

SUBMITTER: Björn Nilsson 

PROVIDER: E-GEOD-75333 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Hematopoietic Age at Onset of Triple-Negative Breast Cancer Dictates Disease Aggressiveness and Progression.

Marsh Timothy T   Wong Irene I   Sceneay Jaclyn J   Barakat Amey A   Qin Yuanbo Y   Sjödin Andreas A   Alspach Elise E   Nilsson Björn B   Stewart Sheila A SA   McAllister Sandra S SS  

Cancer research 20160407 10

Triple-negative breast cancer (TNBC) is considered an early onset subtype of breast cancer that carries with it a poorer prognosis in young rather than older women for reasons that remain poorly understood. Hematopoiesis in the bone marrow becomes altered with age and may therefore affect the composition of tumor-infiltrating hematopoietic cells and subsequent tumor progression. In this study, we investigated how age- and tumor-dependent changes to bone marrow-derived hematopoietic cells impact  ...[more]

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