The effect of palmitate on gene expression profile in proliferating myoblasts

ABSTRACT: High-fat diet or exposure to saturated fatty acids affects skeletal muscle growth and function. The aim of the present study was to investigate the effect of palmitate on the transcriptomic profile of mouse C2C12 myoblasts. Global gene expression was evaluated using whole mouse genome oligonucleotide microarrays, and the results were validated through real-time PCR. A total of 4047genes were identified as differentially expressed, including 3492 down-regulated and 555 up-regulated genes during 48-h-exposure to palmitate (0.1 mmol/l). Functional classification showed the involvement of PA-regulated genes in several processes which regulate cell growth. In conclusion, palmitate modifies the expression of genes associated with: i) myoblast responsiveness to hormones and growth factors, ii) cy

ORGANISM(S): Mus musculus

SUBMITTER: Zofia Wicik 

PROVIDER: E-GEOD-75378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress