Natural antisense transcripts accumulate for over 70% of maize genes and exhibit genotypic-specific expression patterns

ABSTRACT: Natural Antisense Transcripts (NATs) can regulate gene expression by virtue of their ability to form double-stranded RNA duplexes. To investigate NATs in the maize transcriptome, cDNAs from seedling of two inbred lines (B73 and Mo17) were hybridized to an oligonucleotide microarray designed to validate the expression of in silico detected NATs and to screen for NATs that can anneal to a random set of 3’ UTRs and selected repeats found in 3’ UTRs regions. Quantitative Real-Time PCR experiments were conducted to determine the minimum detection threshold of microarray experiment and to thereby identify genes for which both sense and antisense transcripts accumulate to detectable levels. Two independent approaches, strand-specific RT-PCR and S1 nuclease assays were conducted to validate th

ORGANISM(S): Zea mays

SUBMITTER: Patrick Schnable 

PROVIDER: E-GEOD-7608 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress