Systematic reconstruction of molecular cascades regulating growth plate development using single-cell RNA-seq

ABSTRACT: The growth plate, which comprises sequentially differentiated cell layers, is a critical structure for bone elongation and regeneration. Although several key regulators in growth plate development have been identified using primarily genetic perturbation, the systematic understanding is still limited. Here we used single cell RNA-seq to interrogate gene expression profiles of 217 single cells from growth plates, and developed the bioinfromatics pipeline Sinova to de-novo reconstruct physiological growth plate development in both temporal and spatial high-resolution. Our unsupervised model not only confirmed prior knowledge but also enabled systematic discovery of novel genes, potential signal pathways and surface markers CD9/CD200 to precisely depict the development. Sinova further identif

ORGANISM(S): Mus musculus

SUBMITTER: Haofei Luo 

PROVIDER: E-GEOD-76157 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress