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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Neuronal and glial Gene expression from control and healthy patient iPSC-derived forebrain neurons (A)

ABSTRACT: Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total AÎ² secretion profiles were presented here. Single living human iPSC-derived forebrain neuronal cells with variety of sAPPa or total AÎ² secretion profiles were selected and examined for gene expression.

ORGANISM(S): Homo sapiens

SUBMITTER: Tracy Young-Pearse

PROVIDER: E-GEOD-76545 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neuronal and glial Gene expression from control and healthy patient iPSC-derived cortical neurons (B)

Project description:Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total AÎ² secretion profiles were presented here. Single living human iPSC-derived forebrain neuronal cells with variety of sAPPa or total AÎ² secretion profiles were selected and examined for gene expression.

2016-01-06 | E-GEOD-76547 | biostudies-arrayexpress

Neuronal and glial Gene expression from control and healthy patient iPSC-derived cortical neurons

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Design, characterization, and use of a novel amyloid Î²-protein control for assembly, neurotoxicity, and gene expression studies

Project description:A key pathogenic agent in Alzheimerâs disease (AD) is the amyloid Î²-protein (AÎ²), which self-assembles into a variety of neurotoxic structures. Establishing structure-activity relationships for these assemblies is critical for proper therapeutic target identification and design. We examined the effects of AÎ² monomers, dimers, higher-order oligomers, and fibrils on gene expression in primary rat hippocampal neurons. As opposed to âreverseâ AÎ² or non-AÎ² peptides typically used as controls in such studies, we designed novel scrambled AÎ² peptides predicted to behave distinctly from native AÎ². Significant changes in gene expression were observed for all peptide assemblies, but fibrils induced the largest changes. Significant changes in gene expression were observed for all peptide assemblies, but fibrils induced the largest changes. Weighted gene co-expression network analysis (WGCNA) revealed two predominant gene modules related to AÎ² treatment. Many genes within these modules were associated with inflammatory signaling pathways We examined the effects of AÎ² monomers, dimers, higher-order oligomers, and fibrils on gene expression in primary rat hippocampal neurons. Novel scrambled AÎ² peptides, as opposed to âreverseâ AÎ² or non-AÎ² peptides, were used as controls. Please note that the 'XL42_1' sample was excluded from data processing as an outlier (resulting total 23 sample records). However the 'XL42_1' raw data is provided in the 'non_normalized.txt' (total 24 data columns).

2016-08-24 | E-GEOD-85952 | biostudies-arrayexpress

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Project description:The objectives of this study were to assess differences in Bone Marrow Derived Menenchymal Stromal Cells (MSCs) during co-culture with myeloma cells, and to assess differences in myeloma patient MSCs compared to normal donor MSCs. In the study presented here, a Bone Marrow Derived Menenchymal Stromal Cells (MSCs) were analyzed after FACS sorting from 2 week culture in osteogenic media lacking dexamethasone in 3D silk scaffold matrices either in co-culture with the multiple myeloma cell line GFP+Luc+MM1.S or Alone, as controls. Also, monocultures of MSCs grown in 2D, in MSC expansion media, from Normal Donor Controls (ND) or Multiple myeloma patients (MM) were analyzed. Analysis was done looking at microRNA expression in samples with the nanoString microRNA platform for 800 microRNAs.

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