Genome wide map of long noncoding RNA Tug1 binding sites in cultured mouse podocytes

ABSTRACT: We generated a genome wide map of instances where the long noncoding RNA, Tug1, binds to DNA in cultured mouse podocytes under normal glucose conditions using Chromatin-RNA Precipitation coupled with high throughput sequencing (ChIRP-Seq) 48 alternating (even, odd) biotynilated probes were designed to span the full length of Tug1 RNA. Chromatin was prepared from gluteraldehyde crosslinked nuclei from early passage podocytes. Chromatin extracts were duplicated with either even or odd probes. Duplicate samples for Input DNA, Even pulldown (PD) and Odd PD DNA was purified following incubation and supplied for Illumina sequencing by ArrayStar (Rockville, MD).

ORGANISM(S): Mus musculus

SUBMITTER: Shawn Badal 

PROVIDER: E-GEOD-77493 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress