Transcription profiling of leukemia samples prepared using different RNA isolation procedures

ABSTRACT: Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. For the first time, we present a study focused on analyzing the impact of different RNA preparation procedures on gene expression data for different subtypes of pediatric acute leukemias. Experiment Overall Design: We systematically assessed the impact of three different total RNA isolation procedures on variation in expression data: method A: lysis of mononuclear cells, followed by lysate homogenization and RNA extraction; method B: organic solvent based RNA isolation, and method C: organic solvent based RNA isolation followed by purification.

ORGANISM(S): Homo sapiens

SUBMITTER: Andrea Zangrando 

PROVIDER: E-GEOD-7757 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress