Genome-wide analysis of TIAR RNA ligands in mouse macrophages before and after LPS stimulation

ABSTRACT: TIA-1 related protein (TIAR) is a RNA-binding protein involved in several steps of gene expression such RNA splicing and translation. TIAR contains three RNA recognition motifs (RRMs) allowing its interaction with specific sequences localized in the untranslated regions (UTRs) of several mRNAs. In myeloid cells, TIAR has been shown to bind and regulate the translation and stability of various mRNAs encoding proteins important for the inflammatory response, such as TNFα, Cox-2 or IL-8 . Here, we generated two macrophage-like RAW 264.7 cell lines expressing either a tagged full-length TIAR protein or a RRM2-truncated mutant unable to bind RNA with high affinity . By a combination of RNA-IP and microarray analysis (RIP-chip), we identified mRNAs specifically bound by the full-length protei

ORGANISM(S): Mus musculus

SUBMITTER: Yacine Kharraz 

PROVIDER: E-GEOD-77577 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress