Base J represses genes at the end of polycistronic gene clusters in Leishmania major by promoting RNAP II termination

ABSTRACT: Base J and H3.V promote RNA Polymerase (RNAP) II termination within polycistronic gene clusters in the kinetoplastid species Trypanosoma brucei. Although base J has been shown to promote RNAP II termination in the related kinetoplastid species Leishmania major and Leishmania tarentolae, the role of H3.V was unclear. The effect of acute J loss on mRNA transcript abundance was also unknown. We find here that H3.V does not promote transcription termination in Leishmania major, but loss of H3.V does reduce J levels. The J loss in H3.V knockout cells is not enough to result in a termination defect, which we show is due to a threshold level of J that is sufficient to promote termination. Loss of J beyond that threshold results in termination defects. Further, the decreased J in H3.V knockout cel

ORGANISM(S): Leishmania major

SUBMITTER: Robert Schmitz 

PROVIDER: E-GEOD-77632 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress