Next generation sequencing of Desulfovibrio vulgaris Hildenborough in the presence of Free Nitrous Acid stress

ABSTRACT: Purpose: Free nitrous acid (FNA) has recently been demonstrated as an antimicrobial agent to a range of microorganisms. However, its antimicrobial mechanism is largely unknown. For this study Desulfovibrio vulgaris Hildenborough was selected to gain a systematic understanding of the antimicrobial mechanism of FNA. Methods: D. vulgaris was grown under anaerobic denitrifying conditions and when FNA was added (4 μg-N/L) growth temporarily stopped. From cultures with and without added FNA, RNA was extracted and the abundance of gene transcripts was detected by sequencing. Transcripts exhibiting ≥1 or ≤-1 fold abundance change in the presence of FNA were designated as differentially expressed. Results:The results imply that FNA exerted multiple antimicrobial effects. Growth of the culture

ORGANISM(S): Desulfovibrio vulgaris

SUBMITTER: Shuhong Gao 

PROVIDER: E-GEOD-78834 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress