Next generation sequencing of Pseudomonas aruginosa PAO1 in the presence of Free Nitrous Acid stress

ABSTRACT: Purpose: Free nitrous acid (FNA) has recently been demonstrated as an antimicrobial agent to a range of microorganisms. However, its antimicrobial mechanism is largely unknown. For this study Pseudomonas aeruginosa PAO1 was selected to gain a systematic understanding of the antimicrobial mechanism of FNA. Methods: PAO1 was grown under anaerobic denitrifying conditions and when FNA was added (0.1 mg-N/L) growth temporarily stopped. From cultures with and without added FNA, RNA was extracted and the abundance of gene transcripts was detected by sequencing. Transcripts exhibiting ≥2.5 or ≤-2.5 fold abundance change in the presence of FNA were designated as differentially expressed. Results:In response to FNA, 167 genes showed increased transcription while 424 exhibited deceased transcript

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Shuhong Gao 

PROVIDER: E-GEOD-73323 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress