Localization of RNAPII and 3’ end formation factor CstF subunits on C. elegans genes and operons [ChIP-seq]

ABSTRACT: Transcription termination is mechanistically coupled to pre-mRNA 3’ end formation to prevent transcription much beyond the gene 3’ end. C. elegans, however, engages in polycistronic transcription of operons in which 3’ end formation between genes is not accompanied by termination. We have performed RNA polymerase II (RNAPII) and CstF ChIP-seq experiments to investigate at a genome-wide level how RNAPII can transcribe through multiple poly-A signals without causing termination. Our data shows that transcription proceeds in some ways as if operons were composed of multiple adjacent single genes. Total RNAPII shows a small peak at the promoter of the gene cluster and a much larger peak at 3’ ends. These 3’ peaks coincide with maximal phosphorylation of Ser2 within the C-terminal dom

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Alfonso Garrido-Lecca 

PROVIDER: E-GEOD-79821 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress