A Highly Sensitive and Unbiased Approach for Elucidating Antibody Repertoires

ABSTRACT: Developing B lymphocytes undergo V(D)J recombination to assemble germline V, D, and J gene segments into exons that encode the antigen-binding variable region of immunoglobulin (Ig) heavy (H) and light (L) chains. IgH and IgL chains associate to form the B cell receptor (BCR), which upon antigen binding activates B cells to secrete BCR as an antibody. Each of the huge number of clonally independent B cells expresses a unique set of IgH and IgL variable regions. Ability of V(D)J recombination to generate vast primary B cell repertoires results from combinatorial assortment of large numbers of different V, D, and J segments, coupled with diversification of the junctions between them to generate the complementary determining region 3 (CDR3) for antigen contact. Approaches to evaluate in d

ORGANISM(S): Mus musculus

SUBMITTER: Jiazhi Hu 

PROVIDER: E-GEOD-82126 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress