Unknown,Transcriptomics,Genomics,Proteomics

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Single-Cell RNAseq Reveals That Pancreatic Î²-Cells From Very Old Male Mice Have a Young Gene Signature

ABSTRACT: Aging improves pancreatic Î²-cell function in mice. This is a surprising finding since aging is typically associated with functional decline. We performed single-cell RNA sequencing of Î²-cells from 3 and 26 month old mice to explore how changes in gene expression contribute to improved function with age. The old mice were healthy, had reduced blood glucose levels and increased Î²-cell mass, which correlated to their body weight. Î²-cells from young and old mice had similar transcriptome profiles. In fact, only 193 genes (0.89% of all detected genes) were significantly regulated (â¥ 2-fold; false discovery rate < 0.01; normalized counts > 5). Of these, 183 were downregulated and mainly associated with pathways regulating gene expression, cell cycle, cell death and survival as well as cellular movement, function and maintenance. Collectively, our data show that Î²-cells from very old mice have transcriptome profiles similar to those of young mice. These data support previous findings that aging is not associated with reduced Î²-cell mass or functional Î²-cell decline in mice. Single-cell RNA sequencing of mouse pancreatic islet beta cells

ORGANISM(S): Mus musculus

SUBMITTER: Yurong Xin

PROVIDER: E-GEOD-83146 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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