Using AmpliSeq we have performed quantitative analysis of 20,803 genes in Negative control precursor-miR (NC-Pre-miR) and pre-miR-17 transfected Rheumatoid arthritis Synovial Fibroblasts (RASFs)

ABSTRACT: Purpose: The goals of this study are to determine the effect of microRNA-17 overexpression on 20,803 human genes in RASFs using Ion ProtonTM System platform. Human RASFs from two RA patients were transfected with pre-miR-17 or NC-pre-miR for 48 h and total RNA was prepared using miRNeasy kit (Qiagen). Total RNA integrity was checked using an Agilent Technologies 2100 Bio analyzer (Santa Clara, CA). 10 ng of high quality RNA was used to make cDNA for amplification with the Ion AmpliSeq Transcriptome Human Gene Expression kit (ThermoFisher Scientific). The cDNA was subjected to 12 cycles of amplification with panel primers and barcoded with adapters as recommended. Resulting sequencing libraries were quantified by qPCR using SYBR FAST master mix from KapaBiosystems (Wilmington, MA). Sets

ORGANISM(S): Homo sapiens

SUBMITTER: Salahuddin Ahmed 

PROVIDER: E-GEOD-83930 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress