Genotyping of human cell lines with amplification of single cell vs. non amplied products to examime the effect of allele bias during amplification

ABSTRACT: Single-cell human genome analysis using whole-genome amplified product is hampered by allele bias during amplification. Using an oligonucleotide SNP array, we examined the nature of the allele bias and its effect on the chromosomal copy number analysis. Experiment Overall Design: TB106, lymphoblastoid cell line; CMK11-5 and CMK86, cell line; WGA, whole-genome amplified from bulk DNA; SC, whole-genome amplified product from single cell. Genotype and copy number status were compared between non-amplified products and their single cell products.

ORGANISM(S): Homo sapiens

SUBMITTER: Kazuya Iwamoto 

PROVIDER: E-GEOD-8567 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress